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Accession IconE-MEXP-2702

Transcription profiling of Zea mays embryos under camptothecin (CPT) treatment

Organism Icon Zea mays
Sample Icon 8 Downloadable Samples
Technology Badge Icon Affymetrix Maize Genome Array (maize)

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Description
Camptothecin (CPT) is a plant alkaloid that specifically binds topoisomerase I (Topo I) inhibiting its activity and inducing double stranded breaks in the DNA, activating the genotoxic cell responses, and ultimately, it might trigger programmed cell death (PCD). We used microarrays to detail the changes in gene expression during as a consequence of CPT treatment in maize immature embryos. In four independent experiments immature embryos were plated on MS medium supplemented with 50 uM CPT and incubated during three days. Untreated embryos incubated on MS medium were used as controls.
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