Array-based genome-wide transcriptome analysis of IL-4-treated RAW264 macrohage cell line

The role of G-protein-coupled receptors (GPCRs) in the macrophage polarization remains not well understood. In this study, we illuminate the role of Gpr137b, a GPCR expressed strongly in macrophages, in macrophage polarization. We used RAW264 as a mouse macrophage-like cell line and generated Gpr137b-knockout (KO) cell clones using the CRISPR/Cas9 genome editing system. The Gpr137b-KO and wildtype cells were the treated with interleukin-4 (IL-4) to induce M2 polarization, and their gene expression was analyzed using microarrays.

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