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Accession IconGSE11892

A Global View of Gene Activity and Alternative Splicing by Deep Sequencing of the Human Transcriptome

Organism Icon Homo sapiens
Sample Icon 1 Downloadable Sample
Technology Badge IconIllumina humanRef-8 v2.0 expression beadchip

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Description
Transcriptome of HEK and B cells were analyzed by microarray and RNA-Seq parallely. Both platforms were then compared in terms of sensitivity. To assess whether values were a reliable indicator of gene activity, we correlated these values with hypophosphorylated RNA polymerase II (PolIIa) occupancy, used as a landmark of transcription initiation. For HEK, we identified PolIIa islands by chromatin immunoprecipitation and sequencing (ChIP-Seq).
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