Gene expression in circulating mononuclear cells after exposure to ultrafine carbon particles

Peripheral blood samples were collected before (0 hour) and at 24 hours after exposure from healthy subjects who participated in previous controlled exposures to ultrafine carbon particles (UFP, 50 microg/m3) or filtered air (FA)(n = 3 each). The exposure time was 2 hours. RNA from mononuclear cell fraction (>85% lymphocytes) was extracted, amplified and hybridized to Affymetrix HU133 plus 2 microarrays.

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Huang YC, Schmitt M, Yang Z, Que LG, Stewart JC, Frampton MW, Devlin RB