Characterization of Definitive Endoderm formation from HESC and iPSC lines by Microarray analysis

Build and Download Custom Datasets

refine.bio helps you build ready-to-use datasets with normalized transcriptome data from all of the world’s genetic databases.

GSE25557

Homo sapiens

23 Downloadable Samples

Affymetrix Human Gene 1.0 ST Array (hugene10st)

Submitter Supplied Information

Description

HESC-H9 and iPSC lines 3.5, 3.6 and 3.12 were analyzed using Affymetrix microarray before and after Definitive Endoderm (DE) formation. DE was induced using the ActivinA differentiation protocol described by D'Amour et al., 2006 (PMID: 16258519) Clustering analysis of transcripts that were differentially regulated during DE formation indicated that iPSC lines 3.5 and 3.12 differentiate in manner that is highly similar to HESC-H9 cells iPSC line 3.6 had a more divergent transcriptional profile.

PubMed ID

21151107

Publication Title

Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro.

Total Samples

Submitter’s Institution

Cincinnati Children's Hospital Medical Center

Authors

Spence JR, Mayhew CN, Rankin SA, Kuhar MF, Vallance JE, Tolle K, Hoskins EE, Kalinichenko VV, Wells SI, Zorn AM, Shroyer NF, Wells JM

Source Repository

Gene Expression Omnibus (GEO)

GSM628213

iPSC 3.5_Definitive Endoderm Induced_biological rep 3

GSM628202

iPSC 3.12_Undifferentiated_biological rep 1

GSM628216

iPSC 3.12_Definitive Endoderm Induced_biological rep 3

GSM628217

iPSC 3.6_Definitive Endoderm Induced_biological rep 1

GSM628211

iPSC 3.5_Definitive Endoderm Induced_biological rep 1

GSM628197

H9_Undifferentiated_biological rep 1

GSM628207

iPSC 3.6_Undifferentiated_biological rep 3

GSM628198

H9_Undifferentiated_biological rep 2

GSM628219

iPSC 3.6_Definitive Endoderm Induced_biological rep 3

GSM628206

iPSC 3.6_Undifferentiated_biological rep 2