siPools: highly complex but accurately defined siRNA pools eliminate Off-target effects (HuGene-1_0 ENST)

Short interfering RNAs (siRNA) are widely used as tool for gene inactivation in basic research and therapeutic applications. One of the major shortcomings of siRNA experiments are sequence-specific Off-target effects. Such effects are largely unpredictable because siRNAs can affect partially complementary sequences and function like microRNAs (miRNAs), which inhibit gene expression on mRNA stability or translational levels.

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Hannus M, Beitzinger M, Engelmann JC, Weickert MT, Spang R, Hannus S, Meister G