github link
Accession IconGSE62373

Age-associated Changes in Basal NF-B Function in Human CD4+ T Lymphocytes via Dysregulation of PI3 Kinase (dataset 3)

Organism Icon Homo sapiens
Sample Icon 45 Downloadable Samples
Technology Badge IconIllumina humanRef-8 v2.0 expression beadchip

Submitter Supplied Information

Description
Immune impairment and high circulating level of pro-inflammatory cytokines are landmarks of human aging. However, the molecular basis of immune dysregulation and the source of inflammatory markers remain unclear. Here we demonstrate that in the absence of overt cell stimulation, gene expression mediated by the transcription factor NF-B is higher in purified and rested human CD4+ T lymphocytes from older compared to younger individuals. This increase of NF-B -associated transcription includes transcripts for pro-inflammatory cytokines such as IL-1 and chemokines such as CCL2 and CXCL10. We demonstrate that NF-B up-regulation is cell-intrinsic and mediated in part by phosphatidylinositol 3-kinase (PI3K) activity induced in response to metabolic activity, which can be moderated by rapamycin treatment. Our observations provide direct evidence that dysregulated basal NF-B activity may contribute to the mild pro-inflammatory state of aging.
PubMed ID
Total Samples
45
Submitter’s Institution

Samples

Show of 0 Total Samples
Filter
Add/Remove
Accession Code
Title
Sex
Age
Specimen part
Treatment
Processing Information
Additional Metadata
No rows found
Loading...