The tumor heterogeneity of high grade serous ovarian cancer identified by stem cloning technique

Little is known about a relationship between intratumor heterogeneity and drug resistant ability in high grade serous ovarian cancer. Using stem cell cloning technique on high grade ovarian cancer, we have cloned ovarian cancer colonies at high efficiency. The heterogeneity of ovarian cancer is recapitulated in cloned cancer colony library, and Taxol treatment (100 nM 3 hrs) has been conducted on cancer library and obtained drug resistant cancer clones in vitro. Using cloned original cancer colonies and drug resistant cancer colonies, we have studied the effect of intratumor heterogeneity on acquisition of drug resistance.

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