The effects of Yariv-reagent on barley aleurone GA signaling

Microarray analysis was performed to know how many gibberellin (GA)-responsive genes are inhibited by beta-Yariv reagent, a specific binder of plant arabinogalactan-proteins. cRNAs were prepared from mRNAs isolated from aleurone protoplasts that were treated with GA, GA plus beta-Yariv reagent, or mock (DMSO)-treated for 24 hours, and were subjected to microarray analysis. The analysis was performed twice using target cRNAs prepared independently.

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Mashiguchi K, Urakami E, Hasegawa M, Sanmiya K, Matsumoto I, Yamaguchi I, Asami T, Suzuki Y