Escherichia coli str. K-12 substr. MG1655 Transcriptome or Gene expression

The goal was to establish a robust and scalable RNA-seq process applicable to cultured bacteria as well as to complex community transcriptomes. To this end, we evaluated rRNA depletion methods and chose a protocol that eliminates rRNA reads efficiently and robustly, and largely irrespective of the quality of the RNA input sample.

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