Optimized siRNAs with a minimal off-target effect facilitate the screening of essential genes in cancer cells

siRNAs with dN are a promising and easy approach to characterize essential genes by minimizing the off-target effect in cancer cells. Overall design: mRNA profiles of HCT 116 cells after the transfection of siRNAs or blank control by deep sequencing, using HiSeq 2000

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Li C, Liu Z, Yang F, Liu W, Wang D, Dong E, Wang Y, Wu CI, Lu X