Transcriptome-wide mapping of cut sites of the viral endoribonuclease SOX from Kaposi''s sarcoma-associated herpesvirus (KSHV)

Purpose: identify sites in endogenous mRNAs that are cut by KSHV SOX; Method: parallel analysis of RNA ends (PARE, following Zhai et al., 2014); Results: SOX cuts at discrete locations in mRNAs Overall design: human Xrn1 was knocked down in HEK293T cells by shRNAs or siRNAs to stabilize degradation fragments with free 5'' ends; GFP-SOX or GFP were transfected for ~24 hrs; total RNA samples were collected and subjected to PARE protocol (Zhai et al., 2014)

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Gaglia MM, Rycroft CH, Glaunsinger BA