The acute cold response of brown adipose tissue analyzed by RNA-seq

We applied RNA sequencing (RNA-seq) to map the global changes in gene expression of interscapular brown adipose tissue (iBAT) of mice subjected to acute cold exposure for 3 days. Here we find extensive changes in the iBAT transcriptome in response to cold with a prominent induction of genes associated to lipid-related metabolic processes. Overall design: RNA-seq of poly-A enriched RNA isolated from brown adipose tissue of 5 mice housed at room temperature (22°C) and 5 mice exposed to cold (4°C) for 3 days.

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Marcher AB, Loft A, Nielsen R, Vihervaara T, Madsen JG, Sysi-Aho M, Ekroos K, Mandrup S