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Accession IconSRP062085

Association of Taf14 with acetylated histone H3 directs the DNA damage response and gene transcription

Organism Icon Saccharomyces cerevisiae
Sample Icon 12 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

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Description
We sequenced mRNA from triplicate log-phase cultures of BY4741 (WT) transformed with pRS313-HA3-SSN6 and taf14D transformed with pRS313-HA3-SSN6 (empty vector), full-length pRS313-TAF14-HA3-SSN6, or pRS313-taf14W81A-HA3-SSN6 cultured in synthetic complete media lacking histidine. Overall design: Examination of changes in gene expression when the YEATS domain of Taf14 is mutated so it cannot bind acetyl-H3.
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12
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