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Accession IconSRP066910

Temporal transcriptome analysis of control and Zeb2 knockout mESC in pluripotency and in neural differentiation

Organism Icon Mus musculus
Sample Icon 18 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

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Description
To capture the Zeb2-dependent transcriptional changes in early cell state/fate decisions we performed RNA-seq on Zeb2 control and Zeb2 knockout cells. We chose three stages, which correspond in control ESCs to the naive pluripotent state (d0; very low amounts of Zeb2 mRNA), multipotent progenitors (d4, low Zeb2 mRNA/protein) and early neural progenitors (d6, high Zeb2 mRNA/protein), respectively. Overall design: Three biological replicates of Zeb2 control (Ctrl) and Zeb2 knockout (KO) samples on day 0, day 4 and day 6 of neural differentiation were used in this study (18 samples in total)
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18
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