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Accession IconSRP113763

RNAseq of CD45+ cells excluding mast cells from the skin of K14HPV16 Mcpt5-Cre- and K14HPV16 Mcpt5-Cre+ mice

Organism Icon Mus musculus
Sample Icon 101 Downloadable Samples
Technology Badge IconIllumina HiSeq 2500

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Description
The experiment aimed at determing the influence of mast cell deficiency on the transcriptome of skin-infiltrating leukocytes in K14HPV16 mice at 2month and 6month of age. Overall design: Skin-inflitrating leucocytes were FACS-purified from mast cell proficient (Mcpt5-Cre-) and mast cell deficient (Mcpt5-Cre+) K14HPV16 mice. Mast cells (CD117 high, FCeR1 high) were excluded from the sorting gate. In order to control for minimal mast cell contamination during sorting from K14HPV16 Mcpt5-Cre- skin, mast cell signature transcripts were identified by comparing transcriptomes of samples fromK14HPV16 Mcpt5-Cre- mice in which mast cells were flow cytometrically included vs excluded.
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102
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No associated institution

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