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Accession IconSRP114682

Single cell RNA Seq data of BMDM from mouse infected with Salmonella [hundred_ten_singles]

Organism Icon Mus musculus
Sample Icon 96 Downloadable Samples
Technology Badge IconIllumina HiSeq 2500

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Description
The interaction between a pathogen and a host is a highly dynamic process in which both agents activate complex programs. Here, we introduce a single-cell RNA-Seq method (scDual-Seq) that simultaneously captures both host and pathogen transcriptomes and use it to study the process of infection of individual mouse macrophages with the intracellular pathogen Salmonella typhimurium. Among the infected macrophages, we found three subpopulations and we show evidence for a linear progression through these subpopulations, supporting a model in which these three states correspond to consecutive stages of infection. Overall design: 40 single cells, 6 ten cells bulk, 2 hundred cells bulk, in two time point of infection (0,4 hours after infection)
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96
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No associated institution

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