github link
Accession IconSRP149148

Single-cell RNA-seq of spiral ganglion neurons from wildtype and Vglut3-/- mice

Organism Icon Mus musculus
Sample Icon 226 Downloadable Samples
Technology Badge IconNextSeq 500

Submitter Supplied Information

Description
Molecular heterogeneity among spiral ganglion neurons (SGNs) in the mouse cochlea was investigated in two genetic backgrounds: 1) wildtype, 2) Vglut3-/-, which lack inner hair cell-driven glutamatergic activation of SGNs. Overall design: Individual spiral-ganglion neurons expressing the fluorescent reporter tdTomato were dissociated and manually placed into PCR tubes; single-cell libraries were made by the Smart-seq2 approach; sequencing was done using the NextSeq platform (Illumina) at an average read depth of 4.5 million; bioinformatic analysis was conducted in R. Genotypes: bhlhb5::cre/+; Ai14/+ (wildtype) and bhlhb5::cre/+;Ai14/+; Vglut3-/- (Vglut3-/-). Age: P25-P27
PubMed ID
Total Samples
226
Submitter’s Institution
No associated institution

Samples

Show of 0 Total Samples
Filter
Add/Remove
Accession Code
Title
Subject
Processing Information
Additional Metadata
No rows found
Loading...