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Accession IconSRP178543

Single-cell RNA sequencing on breast cancer cells enriched for cancer stem cell properties using functional assays

Organism Icon Homo sapiens
Sample Icon 121 Downloadable Samples
Technology Badge IconNextSeq 500

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Description
We used mammosphere formation assay and label-retention assay as functional cellular approaches to enrich for cells with different degree of cancer stem cell properties in the breast cancer cell line MDA-MB-231 and performed single-cell RNA sequencing Overall design: Single cells from three different populations: 30 cells from G1 cell cycle phase cultured in adherent conditions, 46 cells with low proliferation cultured in non-adherent conditions (mammosphere assasy), 45 cells with high proliferation cultured in non-adherent conditions (mammosphere assay)
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121
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