BP and ER encode proteins that act synergistically to regulate Arabidopsis inflorescence architecture. To search for genes/proteins that influence the BP/ER signaling pathways, we conducted mutagenesis of the bp er double mutant and found that a mutation in FILAMENTOUS FLOWER (FIL) suppresses many of the morphological/developmental defects in bp er. Given that FIL encodes a Zn-finger containing transcription factor, microarray analysis was conducted on bp er vs. the bp er fil line to identify genes that are misregulated and which might implicate specific genes/proteins/pathways that are involved in regulating inflorescence development.
A novel Filamentous Flower mutant suppresses brevipedicellus developmental defects and modulates glucosinolate and auxin levels.
No sample metadata fields
View SamplesA chemical screen was performed in search of compounds that modify plant responses to sucrose. This screen uncovered that sulfamethoxazole (SMX), a folate biosynthesis inhibitor, acted synergistically with sucrose to inhibit hypocotyl elongation, suggesting interaction between these two pathways. Transcriptome analysis was performed to identify changes in transcript abundance that may underpin crosstalk between sucrose and SMX. Three-day-old dark-grown seedlings were treated to sucrose and SMX at concentrations that induced no change in hypocotyl elongation when administered independently, yet restricted elongation when both were present in the growth media (10mM and 0.2M, respectively). This analysis uncovered multiple core auxin signalling components that exhibit altered transcript abundance in response to co-treatment with sucrose and SMX, suggesting that auxin signalling mediates crosstalk between these two pathways. This study highlights an input through which metabolic status can shape plant growth and development through hormone signalling.
Interplay between sucrose and folate modulates auxin signaling in Arabidopsis.
Specimen part
View SamplesThis SuperSeries is composed of the SubSeries listed below.
Global analysis of della direct targets in early gibberellin signaling in Arabidopsis.
No sample metadata fields
View SamplesThe aim of this study is to identify early DELLA protein-responsive genes using a Dexamethasone (DEX)-inducible system. Two transgenic lines were used: one induces the expression of a dominant, gibberellin non-responsive DELLA protein (rga-delta17); the other is a control line that carries the same vector, but lacks the rga-delta17 transgene. By comparing the gene expression changes in the line that expresses the rga-delta17 protein in the presence or absence of DEX it is possible to identify putative targets of DELLA proteins. An empty vector transgenic line was included in this study to identify genes that might be regulated by the DEX inducible system that are not dependent on the DELLA protein.
Global analysis of della direct targets in early gibberellin signaling in Arabidopsis.
No sample metadata fields
View SamplesThe aim is to identify early gibberellin responsive genes in a gibberellin deficient strain such as ga1-3. Such genes are likely regulated by DELLA proteins which are master gibberellin repressors. DELLA proteins are rapidly degraded after gibberellin treatment, but their direct target genes still need to be elucidated.
Global analysis of della direct targets in early gibberellin signaling in Arabidopsis.
No sample metadata fields
View SamplesArabidopsis seedlings of the wild-type or hormone mutants were treated with plant hormones.
The AtGenExpress hormone and chemical treatment data set: experimental design, data evaluation, model data analysis and data access.
Treatment, Time
View SamplesArabidopsis seedlings of the wild-type were treated with inhibitors.
The AtGenExpress hormone and chemical treatment data set: experimental design, data evaluation, model data analysis and data access.
Treatment, Time
View SamplesAtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana
The AtGenExpress hormone and chemical treatment data set: experimental design, data evaluation, model data analysis and data access.
Specimen part
View SamplesAtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana
The AtGenExpress hormone and chemical treatment data set: experimental design, data evaluation, model data analysis and data access.
Specimen part
View SamplesAtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana
The AtGenExpress hormone and chemical treatment data set: experimental design, data evaluation, model data analysis and data access.
Specimen part
View Samples