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GSE64763
Homo sapiens
77 Downloadable Samples
Affymetrix Human Genome U133A 2.0 Array (hgu133a2)
Description
The molecular etiology of uterine leiomyosarcoma (ULMS) is poorly understood, which accounts for the wide disparity in outcomes among women with this disease. We examined and compared the molecular profiles of ULMS, fibroids, and normal myometrium (NL) to identify clinically relevant molecular subtypes. RNA was hybridized to Affymetrix U133A 2.0 transcription microarrays. Differentially expressed genes and pathways were identified using standard methods.
Publication Title
Molecular subtypes of uterine leiomyosarcoma and correlation with clinical outcome.
Sample Metadata Fields
Sex
View Samples- Homo sapiens
- 95 Downloadable Samples
Description
The human genome produces thousands of long non-coding RNAs (lncRNAs) – transcripts >200 nucleotides long that do not encode proteins. While critical roles in normal biology and disease have been revealed for a subset of lncRNAs, the function of the vast majority remains untested. Here, we developed a CRISPR interference (CRISPRi) platform targeting 16,401 lncRNA loci in 7 diverse cell lines including 6 transformed cell lines and human induced pluripotent stem cells (iPSCs). Large-scale screening identified 499 lncRNA loci required for robust cellular growth, of which 89% showed growth modifying function exclusively in one cell type. We further found that lncRNA knockdown can perturb complex transcriptional networks in a cell type-specific manner. These data underscore the functional importance and cell type-specificity of many lncRNAs. Overall design: 96 RNA-seq samples; 16 ChIP-seq samples
Publication Title
CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells.
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