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SRP089956
Mus musculus
6 Downloadable Samples
Illumina HiSeq 2500
Description
Maternal immune activation (MIA) during pregnancy has been linked to an increased risk in the offspring to develop schizophrenia. In schizophrenic patients, a dysregulation of microglia, the brain''s immune competent cells, was reported and treatment with the immunomodulatory compound minocycline proved to be beneficial. Nevertheless, it remains unclear how specific minocycline treatment acts on microglia cells in vivo. Utilizing a mouse model of schizophrenia triggered by a maternal injection of the viral mimic PolyI:C we performed mRNA-sequencing on freshly isolated microglia form adult PolyI:C mice with and without chronic minocycline treatment. In the adult offspring an altered microglial transcriptome associated with changes in cell activation, motility, adhesion and phagocytosis was accompanied by behavioral deficits. Treatment with 3mg/kg/day minocycline for 5 weeks restored the changes in microglial transcriptional signature and phagocytic activity while attenuating the behavioral deficits. Our findings indicate that maternal immune activation induces profound changes in microglial transcriptome and function in the adult offspring and minocycline represents a valuable drug to restore a normal cellular and functional phenotype.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Disease, Cell line, Treatment
View Samples- Mus musculus
- 6 Downloadable Samples
- Illumina Genome Analyzer IIx
Description
mouse pancreatic beta cells were cultured in the presence glucose. RNA was extracted from cells and the transcriptome was profiled.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Homo sapiens
- 134 Downloadable Samples
- IlluminaHiSeq2500, IlluminaHiSeq2000
Description
No description.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 24 Downloadable Samples
- Illumina HiSeq 2500
Description
In the semi-dominant mouse model, Nan (neonatal anemia), heterozygotes suffer hemolytic anemia at birth and throughout life due to a missense mutation (E339D) in transcription factor KLF1 (Krüppel-like factor 1; formerly EKLF, erythroid Krüppel-like factor) Here, we focus on erythropoiesis in the adult spleen. We performed RNAseq in flow-sorted spleen erythroid precursors from adult Nan and WT littermates rendered anemic by phlebotomy as a means to identify global transcriptome changes specific to the Nan KLF1 defect, as opposed to those characterizing anemia generally. We show that (1) expression variation in adult Nan spleen is driven primarily by cell maturation, (2) genotype influences on gene expression are most prominent in late stages of erythroid differentiation when Klf1 expression is highest, (3) Nan-KLF1 produces tissue-specific differential gene expression, and (4) suboptimal stress and basal erythropoiesis with increased reactive oxygen species (ROS) contribute to anemia in adult Nan mice.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line
View Samples- Mus musculus
- 2 Downloadable Samples
- Illumina HiSeq 2500
Description
RNA-seq of Babesia microti isolates and rodent host
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 2 Downloadable Samples
- Illumina HiSeq 2500
Description
RNA-seq of Babesia microti isolates and rodent host
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 2 Downloadable Samples
- Illumina HiSeq 2500
Description
RNA-seq of Babesia microti isolates and rodent host
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 2 Downloadable Samples
- Illumina HiSeq 2500
Description
RNA-seq of Babesia microti isolates and rodent host
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 2 Downloadable Samples
- Illumina HiSeq 2500
Description
RNA-seq of Babesia microti isolates and rodent host
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 1 Downloadable Sample
- Illumina HiSeq 2500
Description
RNA-seq of Babesia microti isolates and rodent host
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples