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Mus musculus
8 Downloadable Samples
Illumina HiSeq 3000
Description
ADARs are RNA editing enzymes that catalyze the deamination of adenosine to inosine in double-stranded RNAs. In mammals, there are two isoforms of ADAR1 including a p110 isoform, which is constitutively and ubiquitously expressed, and a p150 isoform regulated by an IFN-inducible promoter. The mutation in ADAR1 gene causes Aicardi-Goutieres syndrome (AGS), a severe autoimmune disease in human. Furthermore, the significant decrease in RNA-editing activity was found in the p150 isoform mutant associated with AGS. In this study, we will perform transcriptome-wide analysis and identify the targets of ADAR1p150 isoform.
Publication Title
No associated publication
Sample Metadata Fields
Cell line
View Samples- Homo sapiens
- 10 Downloadable Samples
Description
TIA1 and TIAL1 encode a family of U-rich-sequence-specific mRNA-binding proteins (mRBPs) ubiquitously expressed and conserved in metazoans. By PAR-CLIP, we determined that both proteins bind target sites with identical specificity in 3' UTRs as well as within introns proximal to 5' and 3' splice sites. Double knockout (DKO) of TIA1 and TIAL1 increased target mRNA abundance proportional to the number of binding sites and impacted the accumulation of aberrantly spliced mRNAs including the dsRNA-binding protein PRKRA, whose expression was completely blocked and subsequently triggered the activation of the dsRNA-activated protein kinase EIF2AK2/PKR and stress granule formation. Ectopic expression of PRKRA cDNA or knockout of EIF2AK2 in DKO cells rescued this phenotype. Perturbation of maturation and/or stability of additional targets also compromised cell cycle progression. Our study reveals the essential role of a single mRBP family contributing to fidelity of mRNA maturation, translation and RNA stress sensing pathways in human cells.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 2 Downloadable Samples
- Illumina HiSeq 2500
Description
This project aims to delineate the circular RNA complement of mouse brain at age 8-9 weeks
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Cell line
View Samples- Rattus norvegicus
- 1 Downloadable Sample
Affymetrix Rat Genome U34 Array (rgu34a)
Description
Gene expression profiling in rat lumbar spinal cord following ventral root avulsion in the two inbred rat strains.
Publication Title
Genetically determined susceptibility to neurodegeneration is associated with expression of inflammatory genes.
Sample Metadata Fields
Sex, Specimen part, Time
View Samples
SRP023133- Gallus gallus
- 2 Downloadable Samples
- Illumina HiScanSQ, Illumina HiSeq 2000
Description
Transcriptome sequencing was performed for the chicken B-lymphoma DT40 cell line. rRNA-depletion of total RNA was done, a standard Illumina pair-end library was prepared and sequenced on Illumina HiSeq2000 and HiScan2000.
Publication Title
No associated publication
Sample Metadata Fields
Cell line
View Samples- Homo sapiens
- 8 Downloadable Samples
- Illumina HiSeq 4000
Description
Neurons were made from H9 ESCs using a directed differentiation protocol in spinner flasks. After 86 DIV, cells were dissociated and run through the 10X Genomics Chromium single cell RNAseq platform.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Cell line
View Samples- Homo sapiens
- 30 Downloadable Samples
- NextSeq 500
Description
Studies in human innate lymphoid cell (ILC) development are important in understanding the pathophysiology of immune deficiencies and providing insights into the design of immunotherapies for patients with cancer, infection, and autoimmune disease. Currently, it is unclear where and how ILCs develop in humans. The overall goal of our study is to gain a comprehensive understanding of the cellular and molecular components that regulate human ILC development and function in order to best understand how they work in physiological and pathological states.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Danio rerio
- 2 Downloadable Samples
- IlluminaHiSeq2000
Description
Differential Expression between testis and ovary in two months of fish
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Danio rerio
- 2 Downloadable Samples
- IlluminaHiSeq2000
Description
Differential expression between WT and CD82a from Morpholino-treated embyro of Danio rerio
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Arabidopsis thaliana
- 8 Downloadable Samples
- Illumina HiSeq 2000
Description
Based on the pkl dP mutant phenotype, it is predicted that PKL plays a key role in regulating GA-responsive genes that are important for vegetative growth and phase transitions. To test this hypothesis, global transcriptome analysis was performed by RNA-Seq using shoots of 13d-old ga1-13 and ga1-13 pkl that were mock-treated or 10 µM GA3-treated for 24 h.
Publication Title
No associated publication
Sample Metadata Fields
Age, Specimen part
View Samples