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E-MEXP-549
Homo sapiens
21 Downloadable Samples
Affymetrix Human Genome U133A Array (hgu133a)
Description
The purpose of the experiment was to generate a time course of gene expression following irradiation. The goal was then to model this data to extract hidden variables - chiefly, the activity profiles of the p53 transcription factor. Using this information the aim was to predict which transcripts changed by IR were targets of p53. Cells in log phase (1 x 106 ml-1) were ?-irradiated with 5 Gy at room temperature (RT) at a dose rate of 2.45 Gy per minute with a 137Cs ?-irradiator. Cells were harvested at 0, 2, 4, 6, 8, 10 and 12 hours, and RNA and protein were extracted (Trizol, Invitrogen). Affymetrix U133A arrays were hybridized as standard (www.affymetrix.co.uk). Array quality was determined using R and GCOS .rpt file values. The time course was replicated three times from independent cell preparations.
Publication Title
Ranked prediction of p53 targets using hidden variable dynamic modeling.
Sample Metadata Fields
Specimen part, Disease, Cell line, Time
View Samples- Mus musculus
- 18 Downloadable Samples
- Affymetrix Mouse Genome 430 2.0 Array (mouse4302)
Description
Despite Hedgehogs influence on T-cell activation and proliferation, the transcriptional targets of Gli2 in lymphocytes are not known. We therefore examined the Hedgehog-dependent transcriptional response of resting and early-stage activated T-cells in order to define their transcriptional response to Hedgehog pathway activation.
Publication Title
Tissue-derived hedgehog proteins modulate Th differentiation and disease.
Sample Metadata Fields
Specimen part, Treatment
View Samples- Mus musculus
- 56 Downloadable Samples
- Affymetrix Mouse Expression 430B Array (moe430b), Affymetrix Mouse Expression 430A Array (moe430a)
Description
The basic design of the experiment is as described in Fryer et al.(2000)Diabetes 49:1978, and is similar to proto-BAIR (EXP_BAIR_0603_02). H-2Kb muscle cells were treated with metformin (2mM) or insulin (100nM) for 0, 2 or 12 hours.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Cell line, Compound, Time
View Samples- Mus musculus
- 32 Downloadable Samples
- Affymetrix Mouse Expression 430B Array (moe430b), Affymetrix Mouse Expression 430A Array (moe430a)
Description
Mice which lack IRS-2, develop insulin resistance. Comparison between liver tissue from 5 weeks old IRS-2 KO mice and wild type.
Publication Title
Gene expression analysis of liver tissue in five week old Insulin Receptor Substrate-2 (IRS-2) knock out mice.
Sample Metadata Fields
Age, Specimen part
View Samples- Mus musculus
- 28 Downloadable Samples
- Affymetrix Mouse Expression 430B Array (moe430b), Affymetrix Mouse Expression 430A Array (moe430a)
Description
Mice which lack IRS-2, develop insulin resistance. Comparison between muscle tissue from 5 week old IRS-2 KO mice and wild type.
Publication Title
Gene expression analysis of muscle tissue in five week old Insulin Receptor Substrate-2 (IRS-2) KO mice.
Sample Metadata Fields
Age, Specimen part
View Samples- Mus musculus
- 22 Downloadable Samples
- Affymetrix Mouse Expression 430B Array (moe430b), Affymetrix Mouse Expression 430A Array (moe430a)
Description
Mice which lack IRS-2, develop insulin resistance. Comparison between muscle tissue from 8 week IRS2-KO and wild type mice.
Publication Title
Gene expression analysis of muscle tissue in eight week old Insulin Receptor Substrate-2 (IRS-2) knock-out mice.
Sample Metadata Fields
Age, Specimen part
View Samples- Mus musculus
- 22 Downloadable Samples
- Affymetrix Mouse Expression 430B Array (moe430b), Affymetrix Mouse Expression 430A Array (moe430a)
Description
Mice which lack IRS-2, develop insulin resistance. Comparison between liver tissue from 8 week old IRS-2 KO and wild type mice.
Publication Title
No associated publication
Sample Metadata Fields
Age, Specimen part
View Samples- Mus musculus
- 22 Downloadable Samples
- Affymetrix Mouse Expression 430B Array (moe430b), Affymetrix Mouse Expression 430A Array (moe430a)
Description
Mice which lack IRS-2, develop insulin resistance. Comparison between white adipose tissue from 8 week old IRS-2 KO and wild type mice. Processed data is not included for this experiment and therefore these data are not MIAME compliant
Publication Title
Gene expression analysis of white adipose tissue in eight week old Insulin Receptor Substrate-2 (IRS-2) knock out mice.
Sample Metadata Fields
Age, Specimen part
View Samples- Homo sapiens
- 839 Downloadable Samples
Illumina HumanHT-12 V4.0 expression beadchip, Illumina HumanHT-12 V3.0 expression beadchip
Description
This SuperSeries is composed of the SubSeries listed below.
Publication Title
Diagnosis of Kawasaki Disease Using a Minimal Whole-Blood Gene Expression Signature.
Sample Metadata Fields
Sex
View Samples- Homo sapiens
- 202 Downloadable Samples
- Illumina HumanHT-12 V4.0 expression beadchip, Illumina HumanRef-8 v3.0 expression beadchip
Description
This SuperSeries is composed of the SubSeries listed below.
Publication Title
Diagnostic Test Accuracy of a 2-Transcript Host RNA Signature for Discriminating Bacterial vs Viral Infection in Febrile Children.
Sample Metadata Fields
Sex, Specimen part, Disease, Disease stage
View Samples