submitter_institution:Klinikum der Universitt Mnchen Results

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Microarray (1465)

Platform

Affymetrix Mouse Genome 430 2.0 Array (mouse4302) (244)

Affymetrix Human Gene 1.0 ST Array (hugene10st) (236)

Affymetrix Mouse Gene 1.0 ST Array (mogene10st) (185)

Affymetrix Mouse Genome 430A 2.0 Array (mouse430a2) (161)

Affymetrix Arabidopsis ATH1 Genome Array (ath1121501) (142)

MouseRef-8 v2.0 Expression BeadChip (125)

Includes Publication (48)

GSE11898

Expression data from primary mesangial cells stimulated with DNA and RNA ligands

Mus musculus
9 Downloadable Samples
Affymetrix Mouse Genome 430 2.0 Array (mouse4302)

Description

Extrarenal viral infections commonly trigger glomerulonephritis mostly in association with immune complex disease. The immunoglobulin component of immune complexes can activate glomerular cell Fc receptors but whether complexed viral nucleic acids contribute to glomerular inflammation remains unknown. Glomerular mesangial cells express TLR3 but lack TLR7-9, hence, it is unclear whether mesangial cells can recognize and respond to viral ssRNA or DNA. Here we studied the immune responses activated by 3P-RNA (5'Triphosphate RNA) and Non-CpG DNA (Double stranded DNA) in primary mesangial cells (PMC).

Publication Title

Viral RNA and DNA trigger common antiviral responses in mesangial cells.

Sample Metadata Fields

No sample metadata fields

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GSE40301

GPI-anchored Timp1 protein

Homo sapiens
10 Downloadable Samples
Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2)

Description

Tissue inhibitor of metalloproteinase 1 (TIMP-1) controls matrix metalloproteinase (MMP) activity through 1:1 stochiometric binding. Human TIMP-1 fused to a glycosylphosphatidylinositol (GPI) anchor (TIMP-1-GPI) shifts the activity of TIMP-1 from the extracellular matrix to the cell surface. TIMP-1-GPI treated renal cell carcinoma cells (RCC) show increased apoptosis and reduced proliferation. Transcriptomic profiling and regulatory pathway mapping were used to identify potential mechanisms driving these effects. Significant changes in inhibitor of DNA binding (IDs), TGF-1/SMAD and BMP pathways resulted from TIMP-1-GPI treatment. These events were linked to reduced TGF-1 signaling mediated by inhibition of proteolytic processing of latent TGF-1 by TIMP-1-GPI.

Publication Title

No associated publication

Sample Metadata Fields

Specimen part

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GSE64250

ASA404 and TNF treatment in preclinical models of endocrine tumors is associated with differential TNF-R 1 and TLR- 4 signaling

Homo sapiens
7 Downloadable Samples
Affymetrix Human Gene 1.1 ST Array (hugene11st)

Description

Purpose: ASA404 (Vadimezan) belongs to a class of agents with disrupting properties against tumor vasculature, which is partly mediated by TNF-signaling. Our aim was to investigate the potential therapeutic applicability of ASA404 against endocrine tumors. Experimental Design: We determined anti-tumoral effects in preclinical models of neuroendocrine tumors of the gastroenteropancreatic system [1] and adrenocortical cancer (NCI-H295R) by histology and immunohistochemistry. Furthermore, we characterized these models with their clearly different responsiveness regarding TNF synthesis and signaling. Results: Upon treatment of tumor bearing mice significant anti-tumoral effects, an increase in TNF as well as TNF-specific activation of downstream signaling were evident in the BON tumor model while no comparable effects were detectable for NCI-H295R. Two important modulator of TNF-signaling, toll-like-receptor 4 (TLR-4) and its adaptor protein LY96 were found highly expressed in BON tumors and transgenic expression in NCI-H295R partly restored TNF responsiveness. Furthermore, expression of IRAK2-kinase, which has recently been linked to TLR-4-signaling as a mediator of sustained TNF release was induced upon TNF-treatment in BON, but not in NCI-H295R cells. Finally, we identified TNFAIP3/A20, a member of an inhibitory feedback-loop downstream of both investigated signaling cascades, as overexpressed in the adrenocortical carcinoma tumor model. Subsequent analyses of clinical patient samples confirmed a correlation between tumor TNFAIP3 expression levels and overall survival in patients with ACC. Conclusions: Taken together our findings provide evidence that modulation of TNF-signaling could be of relevance both for the clinical course of ACC patients and as a marker of treatment response.

Publication Title

No associated publication

Sample Metadata Fields

Specimen part, Treatment

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GSE43928

Expression data from TNF-stimulated mouse glomeruli

Mus musculus
12 Downloadable Samples
Affymetrix Mouse Genome 430 2.0 Array (mouse4302)

Description

The specific contribution of the two TNF-receptors Tnfr1 and Tnfr2 to TNF-induced inflammation in the glomerulus is unknown. In mice, TNF exposure induces glomerular expression of inflammatory mediators like adhesion molecules and chemokines in vivo, and glomerular accumulation of leukocytes.

Publication Title

Distinct contributions of TNF receptor 1 and 2 to TNF-induced glomerular inflammation in mice.

Sample Metadata Fields

Specimen part, Treatment

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GSE85240

Gene expression changes in stimulated and unstimulated Foxp1-deficient B cells.

Mus musculus
10 Downloadable Samples
Affymetrix Mouse Genome 430 2.0 Array (mouse4302)

Description

Foxp1 is expressed throughout B cell development, but the physiological functions in mature B lymphocytes are unknown. We therefore evaluated differential gene expression in Foxp1-deficient B cells, with or

Publication Title

Foxp1 controls mature B cell survival and the development of follicular and B-1 B cells.

Sample Metadata Fields

Specimen part

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E-MTAB-1036

Transcription profiling of pancreata from Ptf1a-Cre;LSL-KrasG12D and Ptf1a-Cre;LSL-KrasG12D;RelAfl/fl mice

Mus musculus
6 Downloadable Samples
Affymetrix Mouse Genome 430A 2.0 Array (mouse430a2)

Description

Oncogene-induced senescence in early mPanIN lesions depends on intact RelA function in vivo. We investigated the difference in gene expression between KrasG12D pancreata with and without deletion of RelA. Pancreata from 7 day old mice were prepared, RNA was isolated and Affymetrix microarray expression analysis was performed.