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Mus musculus
16 Downloadable Samples
Illumina HiSeq 1500
Description
Mouse hair follicles contain distinct epidermal stem cell populations that reside in their own microenvironments. To understand their molecular identities and surrounding microenvironments, each stem cell compartment was isolated from several different eGFP reporter mouse lines by FACS and their transcriptome data was obtained by RNA sequencing.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Disease
View Samples- Danio rerio
- 12 Downloadable Samples
- IlluminaHiSeq1500
Description
An RNA-seq analysis was performed using zebrafish granule cells, Purkinje cells, IO neurons, and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired-end libraries employing the Quartz-seq method for low amount total RNA.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Homo sapiens
- 8 Downloadable Samples
Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2)
Description
Functional discrimination between normal centroblast and centrocyte obtained from human inflamed tonsils after cell sorting.
Publication Title
CXCR4 expression functionally discriminates centroblasts versus centrocytes within human germinal center B cells.
Sample Metadata Fields
Specimen part
View Samples- Sus scrofa
- 25 Downloadable Samples
- NextSeq 500
Description
RNA-Seq of jejunum for 30 pigs with divergent feed efficiency phenotypes
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part
View Samples- Sus scrofa
- 28 Downloadable Samples
- NextSeq 500
Description
RNASeq of hypothalamus for 30 pigs with divergent feed efficiency phenotypes
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part
View Samples- Homo sapiens
- 18 Downloadable Samples
- Affymetrix Human Transcriptome Array 2.0 (hta20)
Description
Expansion for hematopoietic cells from umbilical cord blood is a strategy for use this cell source in clinic transplants, however, it is important to know about the genomic changes that can occur in expanded cells. In order to detect global expression profiles changes in hematopoietic stem and progenitors cells generated in vitro, we analyzed hematopoietics populations obtained by FACS in fresh from umbilical cord blood. HSC (fHSC) was defined as CD34+ CD38- CD71- CD45RA- Lin- and were cocultured with stromal cell line OP-9 plus FL, SCF, IL3, IL6, TPO, GMCSF and G-CSF by 7 days, after time we repurified HSC population by FACS using same immunophenotype (ivHSC). In other hand, fresh erythroid progenitors cells (fEPC) were identified as CD34+CD38+CD71+CD45RA- Lin- and fresh myeloid progenitors cells (fMPC) were identified as CD34+CD38+CD71-CD45RA+Lin-. In vitro progenitors cells (ivEPC and ivMPC) were obtained by culturing fHSC in Stemspan serum-free media plus SCF, TPO, IL6, FL and IL3 by 10 days, after time cells were repurified by FACS using same immunophenotype for fresh progenitors. In vitro generated cells were compared with their corresponding fresh population cells.
Publication Title
Functional Integrity and Gene Expression Profiles of Human Cord Blood-Derived Hematopoietic Stem and Progenitor Cells Generated In Vitro.
Sample Metadata Fields
Specimen part
View Samples- Homo sapiens
- 13 Downloadable Samples
- Affymetrix Human Gene 1.0 ST Array (hugene10st)
Description
Analysis of aldosterone-producing adenoma (APA) samples from patients with primary hyperaldosteronism. These APAs have a somatic mutation in either KCNJ5, CACNA1D, or ATP1A1. Results provide insight into the different mechanisms each mutation may cause leading to elevated aldosterone production in APA.
Publication Title
Somatic mutations in ATP1A1 and CACNA1D underlie a common subtype of adrenal hypertension.
Sample Metadata Fields
Specimen part, Disease, Disease stage
View Samples- Homo sapiens
- 12 Downloadable Samples
- IlluminaHiSeq2500
Description
46BR.1G1 cell line is impaired in DNA ligase 1 (LIG1) activity resulting in an increased level of endogenous single (SSBs) and double stranded DNA breaks (DSBs). 46BR.1G1 fibroblastoid cells represent a suitable model system to investigate how cells cope with low levels of chronic DNA damage, a condition frequently encountered in tumors. Transcriptional alterations in 46BR.1G1 cells were determined by RNAseq by comparison with 7A3, a cell line in which the defect was rescued by stable expression of ectopic wild-type Lig1. The identification of genes differentially expressed in 46BR.1G1 cells would contribute to the elucidation of DNA damage response (DDR) mechanisms.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples
SRP089956- Mus musculus
- 6 Downloadable Samples
- Illumina HiSeq 2500
Description
Maternal immune activation (MIA) during pregnancy has been linked to an increased risk in the offspring to develop schizophrenia. In schizophrenic patients, a dysregulation of microglia, the brain''s immune competent cells, was reported and treatment with the immunomodulatory compound minocycline proved to be beneficial. Nevertheless, it remains unclear how specific minocycline treatment acts on microglia cells in vivo. Utilizing a mouse model of schizophrenia triggered by a maternal injection of the viral mimic PolyI:C we performed mRNA-sequencing on freshly isolated microglia form adult PolyI:C mice with and without chronic minocycline treatment. In the adult offspring an altered microglial transcriptome associated with changes in cell activation, motility, adhesion and phagocytosis was accompanied by behavioral deficits. Treatment with 3mg/kg/day minocycline for 5 weeks restored the changes in microglial transcriptional signature and phagocytic activity while attenuating the behavioral deficits. Our findings indicate that maternal immune activation induces profound changes in microglial transcriptome and function in the adult offspring and minocycline represents a valuable drug to restore a normal cellular and functional phenotype.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Disease, Cell line, Treatment
View Samples- Homo sapiens
- 275 Downloadable Samples
- Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2)
Description
Tissue samples have been isolated during corornary artery by-pass grafting (CABG)surgery from the atheroscelrotic arterial wall (AAW, aortic root puncture for proxmal ligation of by-pass vessel), non-atherosclertoci arterial wall (NAAW, distal part of mammary artery used a graft for LAD), liver, skeletal muscle (Recturs m), pericardial mediastinal visceral fat) in CAD patients. Carotid lesions samples from 25 validation patients.
Publication Title
Multi-organ expression profiling uncovers a gene module in coronary artery disease involving transendothelial migration of leukocytes and LIM domain binding 2: the Stockholm Atherosclerosis Gene Expression (STAGE) study.
Sample Metadata Fields
Specimen part
View Samples