RNA-Seq of jejunum for 30 pigs with divergent feed efficiency phenotypes
No associated publication
Sex, Specimen part
View SamplesRNASeq of hypothalamus for 30 pigs with divergent feed efficiency phenotypes
No associated publication
Sex, Specimen part
View SamplesExpansion for hematopoietic cells from umbilical cord blood is a strategy for use this cell source in clinic transplants, however, it is important to know about the genomic changes that can occur in expanded cells. In order to detect global expression profiles changes in hematopoietic stem and progenitors cells generated in vitro, we analyzed hematopoietics populations obtained by FACS in fresh from umbilical cord blood. HSC (fHSC) was defined as CD34+ CD38- CD71- CD45RA- Lin- and were cocultured with stromal cell line OP-9 plus FL, SCF, IL3, IL6, TPO, GMCSF and G-CSF by 7 days, after time we repurified HSC population by FACS using same immunophenotype (ivHSC). In other hand, fresh erythroid progenitors cells (fEPC) were identified as CD34+CD38+CD71+CD45RA- Lin- and fresh myeloid progenitors cells (fMPC) were identified as CD34+CD38+CD71-CD45RA+Lin-. In vitro progenitors cells (ivEPC and ivMPC) were obtained by culturing fHSC in Stemspan serum-free media plus SCF, TPO, IL6, FL and IL3 by 10 days, after time cells were repurified by FACS using same immunophenotype for fresh progenitors. In vitro generated cells were compared with their corresponding fresh population cells.
Functional Integrity and Gene Expression Profiles of Human Cord Blood-Derived Hematopoietic Stem and Progenitor Cells Generated In Vitro.
Specimen part
View SamplesHuman immature dendritic cells derived from monocytes were activated to become mature dendritic cells by exposure to platebound Collagen I or Collagen. The importance of OSCAR-signalling was evaluated by pre-incubating the immature dendritic cells with an inhibitory monoclonal antibody to the OSCAR receptor, or an isotype control. As a positive control, immature dendritic cells were activated by using LPS. The set-up was examined at 4 hours and 20 hours to evaluate early and late effects of OSCAR signalling.
Collagen induces maturation of human monocyte-derived dendritic cells by signalling through Osteoclast Associated Receptor
Specimen part, Subject, Compound, Time
View SamplesThe presence of RNA in the spermatozoa collected in HTF medium from cauda epididymis of 12-week-old C57/BL6 male mice.
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Sex, Age, Specimen part, Disease, Cell line
View SamplesMouse hair follicles contain distinct epidermal stem cell populations that reside in their own microenvironments. To understand their molecular identities and surrounding microenvironments, each stem cell compartment was isolated from several different eGFP reporter mouse lines by FACS and their transcriptome data was obtained by RNA sequencing.
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Sex, Age, Specimen part, Disease
View SamplesAn RNA-seq analysis was performed using zebrafish granule cells, Purkinje cells, IO neurons, and glial cells. The transcriptomes were sequenced using Illumina HiSeq with paired-end libraries employing the Quartz-seq method for low amount total RNA.
No associated publication
No sample metadata fields
View SamplesFunctional discrimination between normal centroblast and centrocyte obtained from human inflamed tonsils after cell sorting.
CXCR4 expression functionally discriminates centroblasts versus centrocytes within human germinal center B cells.
Specimen part
View SamplesThis SuperSeries is composed of the SubSeries listed below.
Host Transcription Profile in Nasal Epithelium and Whole Blood of Hospitalized Children Under 2 Years of Age With Respiratory Syncytial Virus Infection.
Sex, Age, Specimen part, Disease, Disease stage
View SamplesAnalysis of transcriptional profiles in whole blood and nasopharyngeal swaps from children hospitalized with lower respiratory tract infections at their admission and their discharge, and diagnosed with either RSV or rhinovirus infections.
No associated publication
Sex, Age, Specimen part, Disease stage
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