Plant volatiles can mediate plant-plant communication in the sense that plants attacked by herbivores can signal their unattacked neighbors of danger by emitting HIPVs. We call this the priming effect. Since the plant defense response is a systematic process involving numerous pathways and genes,to characterize the priming process, a time course study using a genome-wide microarray may provide more accurate information about the priming process. Furthermore, to what extent do the priming process and direct defense share similar gene expression profiles or pathways are also not clear.
Transcriptional analysis of Arabidopsis thaliana response to lima bean volatiles.
Specimen part
View SamplesPlants have developed a complicated resistance system, and they exhibit various defense patterns in response to different attackers. However, the determine factors of plant defense patterns are still not clear. Here, we hypothesized that damage patterns of plant attackers play an important role in determining the plant defense patterns. To test this hypothesis, we selected leafminer, which has a special feeding pattern more similar to pathogen damage than chewing insects, as our model insect, and Arabidopsis thaliana as the response plants. The local and systemic responses of Arabidopsis thaliana to leafminer feeding were investigated using the Affymetrix ATH1 genome array.
Transcriptome response analysis of Arabidopsis thaliana to leafminer (Liriomyza huidobrensis).
Specimen part, Treatment
View Sampleswe treated Homo sapiens UMUC-3 cells with 5-AZA-CdR for 5, 9, 13 and 17 days and employed deep sequencing method to analyze alterations in gene expressions and alternative splicing
Isoform switching and exon skipping induced by the DNA methylation inhibitor 5-Aza-2'-deoxycytidine.
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View SamplesCotton premature leaf senescence often occurred with an increasing frequency in many cotton growing areas and caused serious reduction in yield and quality of cotton has been one of the impontant factors that restrict severely the production of cotton.Our laboratory studies showed chilling stress is the key factor that induced A. alternatia infection, caused Alternaria disease and then lead to cotton leaf senescence, but the molecular mechanism of cotton premature leaf senscence is still unclear.
No associated publication
Specimen part
View SamplesCotton seeds (Gossypium hirsutum cv. CCRI12) were grown in a growth chamber under 29/25C temperature and a 16:8 h light:dark cycle, and water was added every two days. All plants were used in experiments at the 6-7 fully expanded true leaf stage, which occurred 5-6 weeks after sowing. Cotton bollworm (CBW; Helicoverpa armigera) larvae were reared on an artificial diet and maintained at 27 2C, 75 10% relative humidity, and 14:10 h light:dark in the laboratory. For insect treatment, seven H. armigera larvae (third instars) were placed on a group of three plants, which were kept within plastic bags (30 40 cm), until time of harvest, with samples for each time point maintained separately. Undamaged plants maintained under the same conditions were used as controls. Cotton leaves from control plants and plants exposed to H. armigera were harvested at 6 h, 12 h, 24 h, and 48 h after onset of herbivory. For each treatment group and time point, cotton leaves were harvested from the three plants per treatment group and flash frozen in liquid nitrogen. For each time point, three replicate treatments and controls were performed.
No associated publication
Specimen part
View SamplesFormer studies demonstrated that the cembranoid type diterpenes in tobacco has a good antimicrobial, antitumor and neuroprotective activity. Hepatocellular carcinoma has been the world's second largest malignant tumor in recent years. In this study, we isolated cembranoid type diterpenes from tobancco and detected its antitumor activity on hepatoma cell lines, a-2, 7, 11-cyprotermine-4, 6-diol (a-CBD) was used to investigate its antitumor activity on HepG2 cells in vitro.
No associated publication
Sex, Specimen part
View SamplesFormer studies demonstrated that the cembranoid type diterpenes in tobacco has a good antimicrobial, antitumor and neuroprotective activity. Hepatocellular carcinoma has been the world's second largest malignant tumor in recent years. In this study, we isolated cembranoid type diterpenes from tobancco and detected its antitumor activity on hepatoma cell lines, a-2, 7, 11-cyprotermine-4, 6-diol (a-CBD) was used to investigate its antitumor activity on HepG2 cells in vitro.
No associated publication
Sex, Specimen part
View SamplesFormer studies demonstrated that the cembranoid type diterpenes in tobacco has a good antimicrobial, antitumor and neuroprotective activity. Hepatocellular carcinoma has been the world's second largest malignant tumor in recent years. In this study, we isolated cembranoid type diterpenes from tobancco and detected its antitumor activity on hepatoma cell lines, a-2, 7, 11-cyprotermine-4, 6-diol (a-CBD) was used to investigate its antitumor activity on HepG2 cells in vitro.
No associated publication
Sex, Specimen part
View SamplesFormer studies demonstrated that the cembranoid type diterpenes in tobacco has a good antimicrobial, antitumor and neuroprotective activity. Hepatocellular carcinoma has been the world's second largest malignant tumor in recent years. In this study, we isolated cembranoid type diterpenes from tobancco and detected its antitumor activity on hepatoma cell lines, a-2, 7, 11-cyprotermine-4, 6-diol (a-CBD) was used to investigate its antitumor activity on HepG2 cells in vitro.
No associated publication
Sex, Specimen part
View SamplesFormer studies demonstrated that the cembranoid type diterpenes in tobacco has a good antimicrobial, antitumor and neuroprotective activity. Hepatocellular carcinoma has been the world's second largest malignant tumor in recent years. In this study, we isolated cembranoid type diterpenes from tobancco and detected its antitumor activity on hepatoma cell lines, a-2, 7, 11-cyprotermine-4, 6-diol (a-CBD) was used to investigate its antitumor activity on HepG2 cells in vitro.
No associated publication
Sex, Specimen part
View Samples