Comparison of met1 mutant with control
DNA methylation and histone modifications regulate de novo shoot regeneration in Arabidopsis by modulating WUSCHEL expression and auxin signaling
Specimen part, Time
View SamplesComparison of Zea mays mature silk (MS), mature pollen (MP) and unfertilized ovary (UFO).
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Sex, Age, Specimen part, Time
View Samplestranscription profiling of different development stage and different treatment
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View SamplesDespite much knowledge is well-known on the transcriptional and enzymological regulation of many genes in starch synthesis, including SBEb, Waxy, and SUS-SH1 genes, less information is available on their expression profiles as a consequence of their dysfunction. Hence, transcriptional adjustments in response to mutations were required for a better understanding of starch biosynthesis pathway, others metabolism and cell response.
Genome-wide transcriptional analysis of maize endosperm in response to ae wx double mutations.
Specimen part
View SamplesThe aim of this experiment is to identify the candidate genes responsive to cytokinin in Arabidopsis floral development. For this purpose, AtIPT4 gene under the control of the AP1 promoter was transformed into wild type plants of Arabidopsis. The transcriptional profiles of the young inflorescence of AP1::AtIPT4 transformants and wild-type plants are compared.
Cytokinin overproduction-caused alteration of flower development is partially mediated by CUC2 and CUC3 in Arabidopsis.
Specimen part
View SamplesPorcine reproductive and respiratory syndrome caused by porcine reproductive and respiratory syndrome virus (PRRSV) is an infectious disease characterized by severe reproductive deficiency in pregnant sows, respiratory symptoms in piglets, and high mortality. In this study, we employed Affymetrix microarray chip technology to compare the gene expression profiles of lung tissue samples from Dapulian (DPL) pigs (a Chinese indigenous pig breed) and DurocLandraceYorkshire (DLY) pigs after infection with PRRSV. During infection with PRRSV, the DLY pigs exhibited the range of clinical features that typify the disease, while the DPL pigs exhibited only mild signs of the disease. The percentage of CD8+ T cells in the DPL pigs was significantly higher than that in the DLY pigs at 21 days post-infection (dpi) (p< 0.05). Interleukin (IL) 1 beta (IL-1) and IL-2 levels showed significant differences between the DPL and DLY pigs at 0 and 7 dpi (p< 0.01). For IL-10, the DLY pigs had significantly higher values than the DPL pigs at 0 and 7 dpi (p< 0.01). Significant differences were apparent between the DPL and DLY pigs in terms of their tumor necrosis factor-alpha (TNF-) and interferon (IFN)-gamma (IFN-) levels at 0 and 7 dpi (p< 0.01). Microarray data revealed 16 differentially expressed genes in the lung tissue samples from the DLY and DPL pigs (q5%), of which LOC100516029 and LOC100523005 were up-regulated in the PRRSV-infected DPL pigs, while the other 14 genes were down-regulated in the PRRSV-infected DPL pigs compared with the PRRSV-infected DLY pigs. The expression levels of 10 of the 16 genes, namely CCDC84, C6ORF52, THYMOSIN, PRVE, HSPCB, CYP2J2, AMPD3, TOR1AIP2, PTGES3, and ACOX3, were validated by real-time quantitative RT-PCR. This study provides a platform for further investigation of the molecular mechanisms underlying the differential immune responses to PRRSV infection in different breeds or lines of pig.
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Specimen part
View SamplesRNA-sequencing of 7 tissues of maize inbred line Zheng 58: mature silk (MS), mature pollen (MP), mature ovary (MO), and 6-days old seedling (SL), immature silk (IMS), silk at 20min after pollination (20MAP), silk at 3h after pollination (3HAP)
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Specimen part
View SamplesDrosophila midline(mid), encodes a T-box transcription factor that is required for cell fate specification and tissue morphogenesis during a series of developmental processes. However, little is known about the target of Mid.
The T-box transcription factor Midline regulates wing development by repressing wingless and hedgehog in Drosophila.
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View SamplesMultiprotein bridging factor 1(MBF1), a transcriptional co-activator that can regulate transcription by forming a bridge between the TATA-box-binding protein and gene-specific transcription factors. MBF1 is required for organ development and environmental stress responses. However, the targets of MBF1 is unknown.
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View SamplesDrosophila tdf, another name apontic (apt), encodes a bZIP transcription factor that is required for the development of trachea, heart, head and neural system. However, little is known about the target of TDF/Apt.
Evolutionarily conserved transcription factor Apontic controls the G1/S progression by inducing cyclin E during eye development.
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