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GSE39819
Bos taurus
8 Downloadable Samples
Affymetrix Bovine Genome Array (bovine)
Description
Infection of cattle with Mycobacterium bovis causes severe financial hardship in many countries, in addition to presenting a health risk for humans. As an intracellular pathogen, M. bovis, adapted to survive and thrive within the intramacrophage environment. However, little is known about expression patterns in the macrophage, particularly in the bovine host.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Mus musculus
- 37 Downloadable Samples
- Affymetrix Mouse Expression 430A Array (moe430a)
Description
The study was designed in order to identify genes differentially expressed when glucocorticoid signaling is blocked by a glucocorticoid-receptor antagonist (RU486 mifepristone) in the context of brain inflammation induced by bacterial lipopolysaccharide (LPS). LPS is only able to cause murine brain damage in our experimental conditions upon RU486 pre-treatment. Hence, the study may reveal potential candidate genes to mediate neuroprotection or neurotoxicity. Due to the factorial design of the experiment, RU486 main-effect could be dissociated from the effects resultant of RU486/inflammation interaction. In addition, brain dissection was conducted to verify the effects in the brain side ipsilateral or contralateral to the site of intracerebral LPS infusion.
Publication Title
Genes involved in the balance between neuronal survival and death during inflammation.
Sample Metadata Fields
Sex, Age, Specimen part
View Samples- Arabidopsis thaliana
- 8 Downloadable Samples
- Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)
Description
In seedlings, the induction of shade avoidance syndrome (SAS) involves a rapid up-regulation for known shade marker genes and subsequently activates an interacting network of various hormones that will eventually lead to cell elongation. We found that the B-box protein AtBBX24 have positive effects on the SAS (positive regulators). Global expression analysis of col and bbx24 seedlings reveals that a large number of genes involved in hormonal signaling pathways are positively regulated by BBX24 in response to simulated shade.
Publication Title
The transcriptional regulator BBX24 impairs DELLA activity to promote shade avoidance in Arabidopsis thaliana.
Sample Metadata Fields
Specimen part, Treatment
View Samples- Zea mays
- 6 Downloadable Samples
Illumina HiSeq 2500
Description
transcriptional regulation of specific carbon and nitrogen metabolism genes in maize plants exposed to post-silking (PD) drought stress.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Treatment
View Samples- Arabidopsis thaliana
- 8 Downloadable Samples
- Illumina HiSeq 2500
Description
It has been reported that IRE1-bZIP60 pathway is conserved for the signal transduction and gene regulation in response to ER stress including high temperature among human, yeast, Arabidopsis, rice and maize. However, the information of their counterparts in wheat is limited and, especially, the biological relevance of the equivalent pathway contributing to heat tolerance is still ambiguous. In present study, we identified heat stress-induced wheat bZIP60 and investigated its splicing pattern depending on IRE1.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Treatment
View Samples- Zea mays
- 1 Downloadable Sample
- Illumina HiSeq 2500
Description
In this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 ?C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 ?C under dark conditions, the immature embryos were collected, and stored at -70 ?C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Zea mays
- 1 Downloadable Sample
- Illumina HiSeq 2000
Description
In this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 C under dark conditions, the immature embryos were collected, and stored at -70 C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Zea mays
- 1 Downloadable Sample
- Illumina HiSeq 2000
Description
In this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 C under dark conditions, the immature embryos were collected, and stored at -70 C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Zea mays
- 1 Downloadable Sample
- Illumina HiSeq 2000
Description
In this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 C under dark conditions, the immature embryos were collected, and stored at -70 C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Zea mays
- 1 Downloadable Sample
- Illumina HiSeq 2000
Description
In this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 C under dark conditions, the immature embryos were collected, and stored at -70 C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples