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Homo sapiens
12 Downloadable Samples
Illumina HiSeq 4000
Description
Angelica polysaccharide (APS) is one of the major active components isolated from Angelica sinensis. Evidences suggested APS may have therapeutic potential for psoriasis. HaCaT cell line was commonly used as an in vitro model to study psoriasis. In the present study, RNA-sequencing (RNA-seq) was performed to investigate the underlying mechanism of APS. Different concentrations of APS (0mg/mL, 50 mg/L, 100 mg/L and 200mg/L) were incubated with HaCaT cells for 36 h and transcriptome sequenced. Comparison of the gene expression profiles between the CK group (i.e., Control group) and ASP groups revealed dramatic differences. All the differentially expressed genes (DEGs) were then classified into 20 expression profiles by trend analysis. Significant enriched trend clusters were then analyzed. Interestingly, cell proliferation related gene ontology (GO) terms were mostly dispersed in the profile 2 and 17. Based on our functional annotation results and reported literature, authors suspected that SERPINE1, SMAD6 and CTGF, together with TGF-ß, may closely relevant to the anti-proliferation effect of APS.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line, Treatment
View Samples- Homo sapiens
- 2 Downloadable Samples
- IlluminaHiSeq2000
Description
Transcriptome of CALML5-depleted human organotypic epidermis
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Homo sapiens
- 2 Downloadable Samples
- IlluminaHiSeq2500
Description
The goal of the study is to identify genes whose expression are enriched in the progenitor or differentiated layers of human skin, with an ultimate aim to find new molecular regulators of skin development and differentiation.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Homo sapiens
- 2 Downloadable Samples
- IonTorrentProton
Description
The goal of this study is to determine the epidermal genes whose expression is significantly altered by depletion of the gene stratifin (SFN).
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Homo sapiens
- 13 Downloadable Samples
Affymetrix Human Gene 1.0 ST Array (hugene10st)
Description
Little is known about the mechanisms underlying the localization of human melanocytes during embryogenesis, and how the characteristics of melanocytes differ in various body sites. Immunohistochemical studies of biopsy tissue obtained from four different anatomic sites (scalp, back, abdomen, and sole) of 31 aborted fetuses following the approval of the ethics committee for the study of human gene analysis revealed that the melanocyte-associated marker gp100 was expressed earlier in embryogenesis than other melanocyte markers. Human fetal melanocytes are initially localized in the epidermis, and then migrate to the hair buds from the epidermis but not the dermis. In the sole, melanocytes localize in eccrine sweat gland ducts. Cultured fetal melanocytes did not stain positively for any melanocyte markers other than MITF and nestin. When co-cultured with normal human keratinocytes and fibroblasts, fetal melanocytes stained positively for gp100. Gene expression studies indicated that fetal melanocytes were topographically diverse, especially sole-derived melanocytes compared with other melanocytes. Expression of several genes, including CHI3L1 and FGF7, was higher in sole-derived melanocytes. These findings suggest that human fetal melanocytes derived from the sole have different profiles both in vivo and in vitro compared with melanocytes from other sites.
Publication Title
No associated publication
Sample Metadata Fields
Age, Specimen part
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SRP065758- Homo sapiens
- 119 Downloadable Samples
- IlluminaHiSeq2500
Description
Aim to identify the potential relationship between DNA methylation and gene expression.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Drosophila melanogaster
- 66 Downloadable Samples
- Illumina HiSeq 2000
Description
This study crossed Drosophila melanogaster genotypes from four populations to the reference genome line. RNAseq data was then generated to study natural variation in allele specific expression.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line
View Samples- Homo sapiens
- 36 Downloadable Samples
- Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2)
Description
T-cell acute lymphoblastic leukemia (T-ALL) and lymphoma (T-LBL) share common morphologic and immunophenotypic features and are treated with similar therapeutic approaches. Nonetheless, they show distinct clinical presentations suggesting that they may represent two different biological entities. In order to investigate T-LBL and T-ALL biological characteristics we used transcriptional profiling approache. Genome-wide gene expression profiling, performed on 20 T-LBL and 10 T-ALL diagnostic specimens, showed that the two malignancies shared a large fraction of their transcriptional profile while a subset of genes appeared to be differentially expressed in T-LBL versus T-ALL. This gene signature included genes involved in chemotactic responses and angiogenesis which might play a role in the different tumor cell localization suggesting that T-LBL and T-ALL could be two distinct diseases with unique transcriptional characteristics.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Disease
View Samples- Homo sapiens
- 32 Downloadable Samples
- Affymetrix Human Genome U133A Array (hgu133a)
Description
In order to identify genes dysregulated by the aberrant transcriptional activity of RUNX1-RUNX1T1, we used microarrays to determine the effect of this mutation on gene expression during myeloid and erythroid development of normal human progenitor cells.
Publication Title
Transcriptional dysregulation mediated by RUNX1-RUNX1T1 in normal human progenitor cells and in acute myeloid leukaemia.
Sample Metadata Fields
Specimen part
View Samples- Arabidopsis thaliana
- 20 Downloadable Samples
- Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)
Description
Seven-day-old white-light-grown Arabidopsis seedlings were exposed for 15 minutes to polychromatic radiation with decreasing short-wave cut-off in the UV range, transferred back to the standard growth chamber and samples were taken 1 and 6 hours after the start of irradiation.
Publication Title
Genome-wide analysis of gene expression reveals function of the bZIP transcription factor HY5 in the UV-B response of Arabidopsis.
Sample Metadata Fields
Age, Time
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